MGN-3 Research Data

MGN-3

Journal Articles: 

Anti-HIV Activity in Vitro of MGN-3, an Activated Arabinoxylane from Rice Bran

Biochemical AND BIOPHYSICAL RESEARCH COMMUNICATIONS 243, 25-29 (1998)
ARTICLE No. RC978047 

Anti-HIV Activity in Vitro of MGN-3, an Activated Arabinoxylane from Rice Bran 

Mamdooh Ghoneum 

Department of Otolaryngology, Drew University of Medicine and Science, 1621 E. 120th Street, Los Angeles, California 90059

Received December 19, 1997 

MATERIALS AND METHODS 

MGN-3. MGN-3 is an arabinoxylane extracted from rice bran that is treated enzymatically with an extract from Basidiomycetes mycelia. It is a polysaccharide that contains B-1, 4-xylopyronase hemicellulose (Fig.1). MGN-3 is commercially known as Biobran (Daiwa Pharm., Co., Ltd., Tokyo, Japan) 

Complete medium (CM). RPMI-1640 (Sigma) was supplemented with 1% antibiotics (v/v) and 20% (v/v) fetal bovine serum and recombinant IL2.
 

Production of HIV-1 p24 antigen.MNCs from 3 healthy individuals were incubated (37°C) with PHA (5µg/ml) for 3 days and then washed before incubation (37°C, 1hr) with HIV-1 SF strain (HIV-1 p24 of 3000 pg/10⁶ cells). MNCs were then washed 3x with PBS to remove unbound virus. Infected cells were incubated (37°C, 7 days) either with or without MGN-3 at various concentrations (0-100µ/ml), in CM. Half of the medium was changed twice per week with corresponding MGN-3 concentrations. At the end of the incubation period, culture supernatants of HIV-1 infected cells were collected and analyzed for viral production. HIV-1 p24 was measured by antigen capturing ELISA using a commercially available kit (DuPont NEN, Boston, MA) according to the protocol provided by the manufacturer. 

Syncytia formation. A slight modification of Johnson and Walker (3) cell fusion assay was used. Briefly, MNC from 5 AIDS patients were cultured with PHA and in the presence or absence of MGN-3 at various concentrations (0-100µg/ml). HIVI-infected MNC cells were 

Copyright 0 1998 by Academic Press 

All rights of reproduction m any form reserved 

¹Data presented at XI International Conference on AIDS, Vancouver BC, Canada, July 7-12,1996.

Back to MGN-3 index
 
 

---

Support Research-Data.com Free Information 
Click On Our Recommended Sponsors