EFFECT OF MGN-3 ON HUMAN NATURAL KILLER
CELL ACTIVITY AND INTERFERON- y SYNTHESIS IN VITRO.
M. Ghoneum. G.Namatalia and C. Kim. Drew University
of Medicine and Science, Los Angeles, CA 90059, and University of California,
Irvine, CA. 92717
June 2-6, 1996, New Orleans, LA.
MGN-3 is an extract of arabinoxylane from rice bran
that has been enzymatically treated with an extract from Hyphomycetes mycelia.
In this study we investigated the effect. MGN-3 on natural killer (NK)
cell activity and interferon-y (IFN-y) synthesis by peripheral blood mononuclear
MNC was prepared from peripheral blood of healthy
individuals and incubated with various concentrations of MGN-3 for 16 hrs.,
and then NK cell activity was measured by 4 hr. Cr-release assay using
K56 Tumor cells as target NK activity was significantly
enhanced (2-5 fold) by treatment with MGN-3 at 0-100 u g/ml.
In an attempt to investigate the mechanism by which
MGN-3 enhances NK activity, we examined the effect of MGN-3 on IFN-y production
by MNC. Culture supernatants of the cells incubated with MGN-3 were collected
and analyzed for INF-y synthesis by ELISA. INF-y production was increased
We concluded that MGN-3 is
a potent biological response modifier (BRM) and may be useful in immunotherapy
of cancer. MGN-3. was offered by Daiwa Pharmaceutical Co. Ltd. Tokyo-Japan.